1. The bio-process unit operation of concentration, screening, filtration,

    purification, separation, dewatering, drying technique and application

2. The bio-purification operation of concentration, screening, filtration,

    purification, separation, sterilization technique and application  

         Due to the bio-molecules of biologicals, bio-matter and bio substances are

         very small and minute, and the process operation are needed to keep the

         bioactivity for production, Up to now, the conventional unit operation unit

         of concentration, extraction, screening, filtration, purification, separation,

         dewatering, drying technique and design are still hard to do the simple, fast,

         economic, convenient, effective, automatic and continuous concentration,

         extraction, screening, filtration, purification, separation, dewatering, drying

         treatment and application

  [point] to concentrate, extract, screen, filter, purify, separate, dewater and dry the

                     specifc biomolecules of microbes, biologicals, bio-matters, bio-substances up

                     to pure and keep the active biomolecules for production   

       100                 50                                          1                               0.2                            0.1                           0.01                       0.001                  0.0001 

    micron           micron                                micron                   micron                     micron                      micron                    micron              micron

            (SDI: silt density index: included biofoulant, organic foulant, inorganic foulant, silica foulant)

The conventional centrifuge technique, design and application are normally the centrifugal force of 1800~3500, 4500~6000,10000 RPM to concentrate, extract, screen, filter, purify, separate, dewater and dry the biomolecules of microbes, biologicals, bio-matters, bio-substances effectively, however, up to now, they are still not able to breakthrough and overcome the defect of unit operation to concentrate, extract, screen, filter, purify, separate, dewater and dry the specific pure biomolecules of microbes, biologicals, bio-matters, bio-substances effectively  

[point] apply the centrifugal force technique design and application to concentrate,  

                  extract, screen, filter, purify, separate, dewater and dry all the substances of

                  water, liquid and products clear and effectively  

                                                Centrifugal design     

water  liquid  biologicals  product

→

↓  centrifuge

→     →   →

1800~3600 rpm applied to concentrate, extract, screen, purify, separate, dewater, dry all the substances larger than ≧1~5 micron rating particles clear and effectively           4500~6000 rpm applied to concentrate, extract, screen, purify, separate, dewater, dry all the substances larger than ≧0.1~0.01 micron rating particles clear and effectively          ≧10000 rpm applied to concentrate, extract, screen, purify, separate, dewater, dry all the substances larger than ≧100~1000 MW particles clear and effectively

   [benefit]  1. small, simple, convenient, fast, effective and batched unit operation

                            2. suitable for laboratory treatment and application

                            3. small volumes of water, liquid and products can also be done effective  

                                concentration, extraction, screening, filtration, purification, separation,

                                dewatering, drying treatment and application

   [defect] 1. only do batched and small volumes unit operation of water, liquid and

                             products of concentration, extraction, screening, filtration, purification,  

                             separation, dewatering, drying treatment and application

                         2. hard to concentrate, extract, screen, filter, purify, separate, dewater and

                            dry the specific pure biomolecules of microbes, biologicals, bio-matters,

                            bio-substances effectively  

  The conventional membrane separation technique, design and application are

   normally the pores size of SMF/UF/NF membrane to concentrate, extract,

   screen, filter, purify, separate, dewater and dry the biomolecules of microbes,

    biologicals, bio-matters, bio-substances effectively, however, up to now, they

    are still not able to breakthrough and overcome the cake filtration defect of  

    unit operation to concentrate, extract, screen, filter, purify, separate, dewater

    and dry the specific pure biomolecules of microbes, biologicals, bio-matters,

    bio-substances effectively  

   [point] apply the SMF/UF/NF membrane separation technique design and application

                       to concentrate, extract, screen, filter, purify, separate, dewater and dry all the

                       substances of water, liquid and products clear and effectively  

                                            membrane separation design    

                                                                ↓

  [benefit]  1. simple, convenient, fast, effective and batched unit operation

                          2. suitable for laboratory and process treatment and application  

                          3. can be applied to concentrate, extract, screen, filter, purify, separate,

                              dewater and dry the specific pure biomolecules of microbes, biologicals,

                              bio-matters, bio-substances effectively

  [defect]  1. only do batched and small volumes unit operation of water, liquid and

                             products of concentration, extraction, screening, filtration, purification,  

                             separation, dewatering, drying treatment and application

                         2. hard to concentrate, extract, screen, filter, purify, separate, dewater and

                            dry the specific pure biomolecules of microbes, biologicals, bio-matters,

                            bio-substances effectively, automatically and continuously  

The new innovative self-flush filtration, membrane separation technique,

design and application can be applied at the different way for effective 

bio-process operation treatment and application

       a. applied at the bioprocess pre-treatment and post-treatment purification

          treatment for pure bio-products production

            1) The bio-process pre-treatment: to screen, filter and purify the contaminations of

                                                                   impurities, solids and suspended solids larger 

                                                                   than specific biomolecules clear  

                                                                                    pre-treatment                                                                     

         2) The bio-process post-treatment: to screen, filter and purify the contaminations of

                                                              organic matter, chemicals, toxic matter, heavy

                                                              metal smaller than specific biomolecules clear

                                                                                       post-treatment                                                                                          

        b. substituted the conventional centrifuge and membrane separation design

           for effective bioprocess operation treatment and application

       The unit operation of bio-purification technique, design and application of

       concentration, screening, filtration, purification, separation and sterilization

       operation and application are applied to purify and separate the biomolecules

       of bacteria, algae, virus, pyrogenic bacteria, endotoxin, pyrogen out of water,

       liquid and products clear effectively, The application effect are different from  

       the bio-process operation:

       a. The bio-purification operation are applied to purify and separate the

           biomolecules of bacteria, algae, virus, fungi, molds, spores, protozoans,

           endotoxin, pyrogen out of water, liquid and products clear for discarding,

           So it can be destroyed the structure of biomolecules into inactive  

       b. The bio-process operation are applied to concentrate, extract the bio-

           molecules of biologicals, microbes, bio-matter and bio-substances to make

           bioproduct, So it’s needed to keep the biomolecules at activation for

           production  

       Due to the incorrect disinfection, sterilization, filtration, purification, bacteria

       lethal rate, aseptic concept and application, the conventional bio-purification

       operation are hard to purify the water, liquids, products up to bacteria free,

       germ-free, endotoxin free and pyrogen free

       If we can be controlled the following point of bio-purification effect, We are  

       easy to purify the water, liquids, products up to bacteria free, germ-free,

      endotoxin free and pyrogen free

  [point 1] To established the correct bacterial purification and lethal concept,

                        technique, application of sterilization, disinfection and purification

    1. The correct bacterial lethal concept of sterilization and disinfection:

          The active microorganisms are disinfected and sterilized into inactive microorganisms

          at the fixed lethal rate under the specific sterilization and disinfection temperature,

          dosage, concentration and times reaction, the higher sterilization temperature, dosage,

          concentration and the more sterilization times will be increased the microorganisms

           lethal rate effectively

          In factually, the effect of sterilization and disinfection are not able to destroy, disinfect

          and sterilize the active microorganisms into inactive microorganisms completely, after

          sterilization and disinfection, the bacterial residues are still standing within the

          sterilized and disinfected water, liquid and products up to meet aseptic free or not

   2. The correct application of sterilization and disinfection technique:

         To monitor the bacterial contaminations of pre-sterilization and disinfection water,

         liquid and products, and setted up the expected sterilization rating effect under the

         expected sterilization temperature, dosage, concentration and times design to sterilize

         and disinfect the sterilized water, liquid and products up to bacteria free, the residues

         low than 1~100 CFU/ml  

       Example: water purification system, after 3~7days sand filter, carbon, resin cartridges,

                        or 5~1 micron filter cartridges filtration, the purified water are contaminated

                        with 10⁵ CFU/ml bacterial contaminations, The aseptic process operation are

                        needed to set up 3~5 D (99.9~99.999%) sterilization and disinfection design

                        for aseptic free water, liquid and products production

  [point 2] To established the correct purification concept technique, design

                         and application to purify and separate the bacteria, virus, fungi,

                         spores, protozoans, endotoxin, pyrogen clear effectively

    1. The correct purification concept to purify and separate the bacteria, virus,

        endotoxin, pyrogen clear effectively

          apply the pore size smaller than bacteria, virus, endotoxin, pyrogen of aseptic filter

          media to purify the bacteria, virus, endotoxin, pyrogen clear and separate the filtered

          bacteria, virus, endotoxin, pyrogen out of filter media clear for constant aseptic

          filtration purification application

          a. the particles size of bacteria, fungi, pyrogenic bacteria, spores about 0.22~22 micron

          b. the particles size of virus, endotoxin, pyrogen about 0.1~0.001 micron

      2. The correct bio-purification technique, design and application  

         to purify  the bacteria, virus, endotoxin, pyrogen clear effectively

          after filtration to purify the bacteria, virus, endotoxin, pyrogen clear, the 

          filtered bacteria, virus, endotoxin, pyrogen will be purified and separated

          out of filter media clear simultaneously to keep the filter media at aseptic 

          free for further effective filtration purification application

          conventional purification method are applied to keep the filter media clean at aseptic

          free by disassembled, clean and replacement the filter media oftenly and daily; If not,

          the filter media will be becomed into the another bacterial contamination growth bed  

          A)     bacteria       daily disassembled, clean and replacement    bacterial residues       

                                            not daily disassembled, clean and replacement                     bacterial residues

         B)             bacteria       daily disassembled, clean and replacement    bacterial residues

                                                not daily disassembled, clean and replacement    bacterial residues

          C)         virus, pyrogen                  before saturation                  bacterial residues

                    endotoxin,

                                                          After saturation                    bacterial residues